使用CRISPR |
您所在的位置:网站首页 › 多囊肾pkd1 基因杂合突变 › 使用CRISPR |
常染色体显性遗传性多囊肾(ADPKD)是最常见的遗传性肾脏疾病,其特征是在肾脏和其他器官中形成了多个充满液体的囊肿。PKD1和PKD2是分别编码polycystin-1和polycystin-2的两个主要致病基因。在这里,我们报道了使用CRISPR-Cas9技术在PKD1基因中具有杂合或复合杂合突变的两个等基因诱导多能干细胞(iPSC)系的产生。的PKD1 +/-和PKD1 - / -的iPSC维持干细胞样的形态,正常核型,多能性和分化能力,在三种胚层。
"点击查看英文标题和摘要" Generation of PKD1 mono-allelic and bi-allelic knockout iPS cell lines using CRISPR-Cas9 system.
Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disease, characterised by the development of multiple fluid-filled cysts in the kidneys and other organs. PKD1 and PKD2 are the two major causative genes encoding for polycystin-1 and polycystin-2, respectively. Here, we report the generation of two isogenic induced pluripotent stem cell (iPSC) lines with either heterozygous or compound heterozygous mutations in the PKD1 gene using CRISPR-Cas9 technology. The PKD1+/- and PKD1-/- iPSCs maintain stem cell-like morphology, normal karyotype, pluripotency and differentiation capacity in the three germ layers. |
今日新闻 |
推荐新闻 |
CopyRight 2018-2019 办公设备维修网 版权所有 豫ICP备15022753号-3 |